I am planning on building a small clean room for producing mushroom spawn in Costa Rica. The clean room will be in the higher mountains at 8000 feet and has fairly constant temperature year around of between 55 degrees at night and up to 70 degrees in the day. Humidity outside is between 50% and 90%. The room size will be 15’x 10’ with 11’ceilings. There are also two spawn storage closets of 5’x4’. The room is being built with 6” Styrofoam walls with a plaster coating and should have an insulation value of R30 in the walls and floor and R40 in the ceiling. It should be very well sealed from the outside and has triple door entry. The room will have one or two persons working in it a few hours a day. There is a refrigerator, incubator, autoclave and other equipment that will product heat. The goal is to keep the laboratory at a temperature of between 70 and 80 degrees and an RH of between 35%-50%. It should be positive pressurized. All intake and recycled air should pass through HEPA filters. Both the workers and the mushroom spawn produce some CO2 that may need to be exchanged.
I’m trying to learn as much as I can before talking with a HVAC professional so that I can talk intelligently with them and also have a better idea if they know what they are doing (in other areas I’ve found that not all professionals are created equally).
Here are the things that I’m wondering. Can I get by without any heating and A/C, using the heat from the devices in the room to warm it and using outside air to keep it cool? Would I need to get an economizer such as the Honeywell Jade or something similar, or could I just assume that the outside air is cooler and use it when the temperature is to high? Will a small home style humidifier be enough to keep the humidity to the required levels? How much to I need to worry about CO2 levels?